If the conservative model had been correct, how many generations of bacterial cell division would have had to pass before meselson and stahl would have observed evidence of a band in the cscl gradient corresponding to a hybrid dna molecule containing both 14n and 15n?.

Answers

Answer 1

If the conservative model had been correct, No generations; a hybrid would never form had to pass before meselson and stahl would have observed evidence of a band in the cscl gradient corresponding to a hybrid dna molecule containing both 14n and 15n.

What is the Meselson and Stahl experiment about?

The Meselson and Stahl experiment is an experiment in 1958 by Matthew Meselson and Franklin Stahl. Meselson and Stahl through their experiment  demonstrated that DNA replicated semi-conservatively, meaning that each strand in a DNA molecule serves as a template for synthesis of a new, complementary strand

Here's the complete question:

If the conservative model had been correct, how many generations of bacterial cell division would have had to pass before Meselson and Stahl would have observed evidence of a band in the CsCl gradient corresponding to a hybrid DNA molecule containing both 14N and 15N?

A) 1 generation

B) 4 generations

C) More than 10 generations

D) No generations; a hybrid would never form.

Learn more about Meselson and Stahl experiment from:

https://brainly.com/question/14803355?referrer=searchResults

#SPJ4


Related Questions

VERY URGENT I WILL GIVE BRAINLIEST!!!

19. What is the purpose of taxonomic systems?

a. To provide descriptive latin names
b. To maintain a small number of taxa
c. To construct a family tree that predicts how many species may be discovered into the future
d. To provide consistent ways to identify and classify organisms as they are being studied ​

Answers

Answer: d. To provide consistent ways to identify and classify organisms as they are being studied

Explanation:

Taxonomy is a process that describes and helps identify different living organisms. The process involves classification, identification, nomenclature.

For what value of eccentricity, e, would the semi-major axis be the same as the aphelion distance?.

Answers

The idea of a planetary orbit. The planet's distance at epihelian r, an equals a times 1 plus the centricity, and the planet's distance at perihelion p equals a times 1 minus the gravitational potential energy between the two planets. The separation between them as a minus of g times, m 1 m 2.

As a result, we can say that gravitational potential energy is proportional to inversely proportional to separation, or that the ratio of gravitational potential energy at apehelion to perihelium equals perihelium distance over apehelium distance, which equals a times 11 minus a times a times. We get this value as 1 plus e, sorry, 1 minus e over 1 plus, and it equals 0.145. So 1 minus e equals 0.145 minus 0.145 p, or the centricity equals 1 minus 0.145 upon 1 plus 0.145, where the centricity is 0.747.

Learn more about centricity here:

https://brainly.com/question/14089595

#SPJ4

Once a cell completes mitosis, molecular division triggers must be turned off. What happens to mpf during mitosis?.

Answers

The cyclin component of MPF is degraded.

What is mitosis?

During the phase of the cell cycle known as mitosis, duplicated chromosomes are physically split in half to form two new nuclei. Mitosis is a method of cell division that results in the production of cells that are genetically similar and in which the total number of chromosomes is preserved. Since this occurs during mitosis, the process is often referred to as equational division.

The cyclin-Cdk complex is the Maturation-promoting factor (abbreviated MPF, also known as a mitosis-promoting factor or M-Phase-promoting factor). It stimulates the cell cycle's mitotic and meiotic stages. Cyclins and cyclin-dependent kinases (Cdks) are in charge of the cell's progression through the numerous checkpoints.

Cyclin-CDK is a protein complex composed of an inactive catalytic subunit of a protein kinase, cyclin-dependent kinase (CDK), and a regulatory subunit, cyclin.

Synthesized cyclins function as activating proteins and attach to Cdks to create a cyclin-Cdk complex. This compound then functions as a signal for the cell to enter the subsequent phase of the cell cycle.

Inactivation of this same complex is also essential for the transition from mitosis to the G1 phase of cell division. This is accomplished through ubiquitination and proteolytic destruction of cyclin B by the anaphase-promoting complex (APC), a ubiquitin ligase.

Hence, the cyclin component of MPF is degraded.

To know more about mitosis the link is given below:

https://brainly.com/question/29035994?

#SPJ4

Does the temperature increase or decrease with altitude in the stratosphere? What
causes this change in temperature?

Answers

Answer:

In this region the temperature increases with height. Heat is produced in the process of the formation of Ozone and this heat is responsible for temperature increases from an average -60°F (-51°C) at tropopause to a maximum of about 5°F (-15°C) at the top of the stratosphere.

Explanation:

1. Using the Universal Genetic Code Chart, fill in the missing amino acids in the amino acid
sequence for species A in the chart on the next page.
2. Using the Information given, fill in the missing mRNA bases in the mRNA strand for species
B In the chart on the next page.

Answers

The so-called "universal" or "standard genetic code," as we all learnt in high school, is a set of guidelines that specify the relationship between the "20" amino acids and the "bases" (codons) found within mRNA.

How many different amino acids are there in the human genome?

There are 20 frequent amino acids in the human genetic code. Additionally, the codons UGA and UAG, which are often used as stop signals, are used to encode selenocysteine (Sec) и pyrrolysine (Pyl), the twenty-first and twenty-second amino acids, respectively.

Is there a common genetic information for amino acids?

Except for a few prokaryotes, cells with mitochondria, and chloroplasts, every organism, including viruses, bacteria, and humans, has the same genetic code. The dining room table .

To know more about genetic code visit :

https://brainly.com/question/29413263

#SPJ1

A water molecule could move all the way through a plant from soil to root to leaf to air and pass through a living cell only once. This living cell would be a part of which structure?.

Answers

A water molecule could move all the way through a plant from soil to root to leaf to air and pass through a living cell only once. This living cell would be a part of the endodermis structure.

The endodermis can be described as a layer of inner cells that usually surround the vascular bundle.

The endodermis is a constant layer of inner cells that surround a plant. Hence, A water molecule could move all the way through a plant from soil to root to leaf to air and pass through a living cell only once in the endodermis of a cell.

The endodermis is the living cells of the innermost cortical layer.

To learn more about endodermis, click here:

https://brainly.com/question/24988158

#SPJ4

the lining of the marrow cavity.target 1 of 5 2. blankcells that can dissolve the bony matrix.target 2 of 5 3. blanklayers of bone matrix.target 3 of 5 4. blanksmall channels that radiate through the matrix of bone.target 4 of 5 5. blankcells that can build bony matrix.

Answers

Each description should be matched with the correct and appropriate terminology as follows:

Endosteum: the lining of the marrow cavity.Osteoclasts: cells that can dissolve the bony matrix.Lamellae: layers of bone matrix.Canaliculi: small channels that radiate through the matrix of bone.Osteoblasts: cells that can build bony matrix.

What is a cell?

A cell can be defined as the fundamental functional, structural and smallest unit of life, which is found within the body of an organism.

What is a joint?

A joint can be defined as a point where two (2) or more bones in the body of an organism (skeletal system) make contact, so as to facilitate the easy movement of body parts.

In Science, cells that have the ability to dissolve the bony matrix are generally referred to as osteoclasts while cells with the ability to build bony matrix are generally referred to as osteoblasts.

Furthermore, the membrane which acts as a lining of the marrow cavity is generally referred to as endosteum and it is mainly responsible for the following:

Bone developmentBone repairBone remodeling.

Read more on bones here: https://brainly.com/question/12423263

#SPJ1

In which two ways do plant cells use the sugar made in photosynthesis ?

• to produce phosphate groups
• to store inorganic matter
• to supply the plant with energy for life
• to make other types of organic molecules

Answers

Answer:

3 and 4

Explanation:

Sugar made from photosynthesis is broken down to release energy needed for life.

and the sugar can also be used to make other organic molecules such as cellulose.

What would the alleles be for the following organisms?
A pea plant is heterozygous for height. (T)

TT

Tt

tt

None of the above

Answers

Answer:

Tt

Explanation:

Heterozygous has two different alleles.

The prefix "hetero-" means different, or others. The only choice that has two different alleles is Tt, and will be your answer.

Learn more about heterozygous, here:

https://brainly.com/question/3258389

in 1944, 29 reindeer were introduced onto st. matthew island, alaska. the reindeer population reached 6000 individuals in 1963. what was the percent increase in the reindeer population over this time period? please choose the correct answer from the following choices, and then select the submit answer button. answer choices approximately 1084 approximately 314 approximately 99 approximately 20,600

Answers

The percent increase in the reindeer population is calculated to be approximately 99% over the time period from 1944 to 1963.

A percent increase can be calculated using the formula:

percent increase = (increase/original number) × 100

Therefore, first, we determine the increase in the population from 1944 to 1963 by subtracting the population in 1944 from the population in 1963;

increase = 6000 - 29 = 5971

Now the percentage increase can be calculated as follows;

percent increase in population = (increase in population / original population) × 100

percent increase in population = (5971 / 6000) × 100

percent increase in population = 0.9952 × 100

percent increase in population = 99.52% ≈ 99%

Hence, the percent increase in the reindeer population is calculated to be approximately 99%.

To learn more about the percent increase; click here:

https://brainly.com/question/25098379

#SPJ4

plant cells produce hormones that affect plant growth and behavior. cytokinesis and auxins are two important types of plant hormones. together, these hormones control cell division in the plant.if cytokinesis is high and auxin is low, the plant produces stems. when auxin is high and cytokinin is low,roots grow . plant hormones travel around plant in vessels called xylem. hormones also move from cell to cell in plants by diffusion or active transport. where are hormones produced in animals , and how are they transported throughout the body

Answers

Answer:

Hormones get things done. Think of them as chemical messengers that are made in one place in the body and deliver their message in a totally different place in the body. And just like hot sauce, a little goes a long way. Hormones are usually found in very small concentrations, but boy to they pack a punch! We know hormones cause a lot of changes in humans (ah, puberty), but did you know that plants have hormones, too? Plants miss out on all the fun of body hair, acne, and voice changes, but read on to learn about the amazing effects that hormones have on plant growth and development!

Explanation:

oum I hope it helps oum... correct me if I'm wrong

how many million metric tons (mmt) of energy-related co2 were released in 2018.how did this compare with 2008?

Answers

5.27 million metric tons (mmt) of energy-related [tex]CO_{2}[/tex] were released in 2018.

Carbon dioxide ([tex]CO_{2}[/tex]) emissions from all energy-related sources in the United States increased to 5.27 billion metric tons in 2018, a 2.7% increase over 2017. Higher natural gas-related emissions brought on by more severe summer and winter weather as well as a rise in petroleum emissions from transportation, which are tied to a robust economy, were the main causes of the increase.

A data series from the U.S. Energy Information Administration's (EIA) Monthly Energy Review shows that the country's  [tex]CO_{2}[/tex]  emissions have decreased six out of the previous ten years and were 12% lower in 2018 than they were in 2005.

According to early figures made public today by the U.S. Energy Information Administration, energy-related  [tex]CO_{2}[/tex]  emissions in 2008 decreased by 2.8% from the previous year (EIA). In 2008, the Gross Domestic Product (GDP) increased by a negligible 1.1 percent.At the same time, energy consumption fell by 2.2 percent.

Learn to know more about energy release on

https://brainly.com/question/655017

#SPJ1

A balloon is filled with gas.
How does the balloon change when the temperature increases?
Select the words from the drop-down menus that correctly complete the explanation.
As temperature increases, the average speed of gas particles Choose....
the pressure of the gas Choose....
and the size of the balloon Choose...
v
. As a result,

Answers

Answer: the balloon will rise as tempature increases because cold air sinks warm air rises.

Explanation:

Name the process by which soluble food enters the blood, diffusion, fermentation or transpiration?

Answers

Answer:

It is called absorption. Digested food enters the blood or lymph through the small intestine wall by diffusion or active transport

Answer:

Diffusion

Explanation:

Diffusion is the movement of a substance from an area of high concentration to an area of low concentration. It can occur all over the body including the large intestine when food enters to blood stream and in the alveoli in the lungs where gases diffuse into the blood stream. Hope this helps :)

There is a net movement of water into a cell from the surrounding tissue fluid. Is the tissue fluid more or less concentrated than the fluid inside this cell?

Answers

Answer:

Less concentrated as, by osmosis, the water moves up the concentration gradient into area of most concentration.

which of the following sources would likely have mitochondrial DNA but lack nuclear DNA?

a: newly deceased victim
b: highly decomposed skeletal remains
c: biological fluids left at a crime scene
d: hair that has fallen from a suspects head

Answers

The source that would likely have mitochondrial DNA but lack nuclear DNA is highly decomposed skeletal remains; option B.

What is DNA?

DNA is the molecule found in living organisms that contains the genetic information of the living organism.

It is a double-stranded helical structure molecule composed of nucleotide monomers linked by phosphodiester bonds.

The DNA in living organisms is found in the nucleus o the cell as well as in the mitochondria of cells.

The DNA in the nucleus is known as nuclear DNA whereas the DNA in the mitochondria is known as mitochondrial DNA.

Learn more about DNA at: https://brainly.com/question/2131506

#SPJ1

Could someone explain to me why people test for macromolecules in labs at school? Also, I can't find any background information on the lab, where we tested for macromolecules in stomach acid. Could anyone help find any information on the background of this lab?
Thanks in advance to anyone that helps! :)

Answers

Students perform tests of macromolecules in a laboratory to test whether lipids, carbohydrates, and proteins are present in various foods.

What are macromolecules?

A macromolecule is defined as a very large molecule that is important to various processes like protein or nucleic acid. It is made up of covalently bonded atoms. Many macromolecules are polymers of smaller molecules which are called monomers. Carbohydrates, lipids, proteins, and nucleic acids are the four main types of a macromolecule which are necessary for normal function as well as the survival of our body. We can use indicators to test the presence of macromolecules such as lipids, carbohydrates, and proteins in many foods in laboratory. We will be testing for the presence of carbohydrates such as glucose, and sucrose as well as complex carbohydrates such as starch and proteins.

So we can conclude that we can test macromolecules in labs at school to check the presence of lipids, carbohydrates, and proteins in various food items.

Learn more about macromolecules here: https://brainly.com/question/5246898

#SPJ1

What does relatively constant mean?

Answers

having meaning or significance only in relation to something else; not absolute

what are the the independent and control variables in onion cell and cheek cell experiment

Answers

The Independent Variable is the magnification: either scanning electron, or surface.

The Dependent Variable is the microscope

Controlled Variable: The two types of cells: Onion, and the Cheek Cell

What is control variable?

Anything kept constant or constrained in a research study is referred to as a control variable. Despite not being relevant to the study's objectives, this variable is controlled because it might have an impact on the results.

Separately inserted in the glass slide and covered with a coverslip are a thin layer of onion and cheek cells. Colored dye can be used to stain the cheek cells and onion cell layer. Magnification can be changed by the researcher.

To learn more about Control Variable from the given link

https://brainly.com/question/17328868

#SPJ9

What cellular process does a mitochondrion perform for the cell?
A. The synthesis of carbohydrates and lipids
B. The division of nucleic acid in replication
C.
The release of energy for metabolic activity
D. The sorting and packaging of proteins

Answers

Answer:

C. The release of energy for metabolic activity

Every cell in your body came from one fertilized egg cell. What does this tell you about the DNA differences between one body cell and another?

Answers

Answer:

cells are different because they use the same set of genes differently. So even though each of our cells has the same 20,000 or so genes, each cell can select which ones it wants to “turn on” and which ones it wants to keep “turned off”.

In a typical conception, nuclear DNA from both parents is mixed into the fertilized egg. That DNA holds the majority of a person's genes, as well as all the genes responsible for a person's features.

What DNA differences observe in fertilized egg cell?

The mother's mitochondrial DNA is exactly replicated in the egg. Every cell in the body is propelled by and assisted in dividing by this DNA.

Every cell has DNA in its nucleus, which serves as the blueprint for producing a wide variety of proteins within the cell.

Different signals can induce embryonic cells to choose particular DNA sequences, which can later be utilized to make proteins and create various cell types.

Therefore, it is possible for each cell to independently decide which genes to “turn on” and which to stay “shut off.”

Learn more about fertilized egg cell here:

https://brainly.com/question/6660999

#SPJ2

Describe how a decrease in populations in populations of socondary consumers in an ecosystem would impact both the primary consumer level and h primary producer level

Answers

A deceease in the secondary consumers in an ecosystem can impact both primary consumers and primary peoducers. The reason why it impacts primary consumers is because they no longer are pret causing them to over populate. The over population of the primary consumers impacts the primary consumers because now there are too many primary consumers that need food. Eventually the primary producers will become exctinct due to the primary consumers eating the primary producers. When the primary producers die out so will the primary consumers, for their food source is gone.

A decrease in populations of secondary consumers in an ecosystem can have significant impacts on both the primary consumer level and the primary producer level.

What are the negative effects caused due to increase in herbivores?

At the primary consumer level, the number of secondary consumers decreases which increases the number of primary consumers, as there are fewer predators to consume them.

This can put additional pressure on the primary producers because of the presence of more herbivores competing for limited resources. The increase in herbivores results in the depletion of plant populations, which can have negative effects on the entire ecosystem.

At the primary producer level, the decrease in populations of secondary consumers also decreases the number of predators, that allow herbivores to reproduce rapidly putting additional pressure on the primary producers.

Therefore, the decrease in populations of secondary consumers can alter the balance between predator and prey populations thereby disrupting the relationships between different trophic levels.

Learn more about herbivores here:

https://brainly.com/question/7626870

#SPJ2

What biopolymer or macromolecule do you think the major component is?

Answers

Lipids, I don't know if you still need it

Michaela is baking a dessert. She measures 100 mL of vegetable oil by pouring it from the bottle into a measuring cup. Then she pours the vegetable oil into a bowl with the other ingredients.

Which best describes what happened to the vegetable oil during this process?

Answers

The shape of the vegetable oil changed but the volume did not.

What is state of matter?

Solids, liquids, and gases all fall under the category of states of matter. Solids are made up of tightly packed particles. In contrast to gases, where they are scattered, liquids have more particle mobility. Particles in chemistry can be molecules, ions, or even atoms.

It is important to understand that only when matter gains or loses energy will its state change. The vegetable oil is simply poured in this instance from a bottle into a cup, then into a bowl. No energy was gained or lost in the process; the only thing that will change is the oil's shape, as the bowl it is currently in is not the same shape as the bottle.

Hence the correct option is the shape of the vegetable oil changed but the volume did not

To learn more about state of matter from the given link

https://brainly.com/question/16982523

#SPJ9

when can the Go phase occur in the cell cycle

Answers

Go phase or also called as resting state or gap phase is the phase in the cell cycle in which the cell is neither dividing nor preparing for division so it's in a resting phase. Many cells consume most of their time in this phase either at rest or performing assigned duties. The cell get in this phase after it is done dividing or duplicating.

What is the smallest number of molecules of atp and gtp consumed in the synthesis of a protein with 200 residues after mrna synthesis, starting from amino acids? assume that the hydrolysis of ppi is equivalent to the hydrolysis of atp for this calculation.

Answers

The smallest number of molecules are 200 ATP and 400 GTP consumed in the synthesis of a protein with 200 residues after mrna synthesis.

What is mrna synthesis and what is ATP and how many ATP and GTP are consumed?mRNA is one of the RNA of ribosomal, manufacture, and transfer RNA synthesized in nucleus using the nucleotide sequence of DNA as template.ATP is adenosine triphosphate used as energy molecules to process the needed function of human and plant body.In here is asked the smallest number of molecules of ATP and GTP consumed in the synthesis of a protein with 200 residues after mRNA synthesis.The answer would be 200 molecules of ATP and 400 molecules of GTP consumed to synthesize protein.

To know more about mRNA visit :

https://brainly.com/question/12903143

#SPJ4

Of the 6 carbons in a starting molecule of glucose, at the completion of pyruvate oxidation _____ carbons are fully oxidized to _____, while _____ carbons remain in _____.

Answers

Answer:

2 carbons; to CO2; 4 carbons; acetyl groups

Explanation:

which is the ring-like muscle at the base of the stomach that controls the flow of partially digested food from the stomach to the duodenum of the small intestine?

Answers

The pyloric sphincter is the ring-like muscle at the base of the stomach that controls the flow of partially digested food from the stomach to the duodenum of the small intestine.

In the field of human anatomy, we can describe the pyloric sphincter as a type of smooth muscle that is responsible for controlling muscle action for letting food from the stomach to pass into the small intestine.

It is through the pyloric sphincter that food moves into the duodenum part of the small intestine and is further digested and processed. We can say that the pyloric sphincter acts as a muscular valve in between the stomach and the small intestine.  If the pyloric sphincter fails to open and food is not sent to the small intestine, then bloating or vomiting will occur.

To learn more about pyloric sphincter, click here:

https://brainly.com/question/13376200

#SPJ4

control of gene expression in eukaryotic cells occurs at which level(s)? only the transcriptional level epigenetic and transcriptional levels epigenetic, transcriptional, and translational levels epigenetic, transcriptional, post-transcriptional, translational, and post-translational levels

Answers

In eukaryotic cells, gene expression is under the control of Option D epigenetic, transcriptional, post-transcriptional, translational, and post-translational levels.

While genetic alterations can affect which proteins are produced, epigenetic changes impact gene expression and can "turn on" and "turn off" genes. The relationship between your genetic, actions, and environment is simple to understand since your environment and habits, such nutrition and exercise, can cause changes in your epigenome. Epigenetic mechanisms are molecular processes that control how an organism's genome is regulated by its environment. The genetic information is transferred from the archival copy of DNA to short-lived messenger RNA during transcription, translation, and subsequent protein modification, which typically results in the synthesis of protein. Despite the fact that all cells in an organism have fundamentally the same DNA, different cell types and functions result from qualitative and quantitative variations in gene expression as well as control of gene expression.

Learn more about epigenetic here:

https://brainly.com/question/2670031

#SPJ4

A good team member will actively listen, which means they will ______. a. listen while looking at the notes they are taking b. constantly look for new things to listen to c. use body language to understand how a speaker feels d. listen to multiple people all at once please select the best answer from the choices provided a b c d

Answers

A good team member will actively listen, which means using body language to understand how the speaker is feeling.

What is Active listening?

Active listening is a skill that can be learned with consistent practice. In active listening, a person employs all of his or her senses. The person responds to the speaker through verbal and nonverbal means (such as nodding and maintaining eye contact), encouraging the speaker to continue speaking. Positive feedback in the form of body language makes the speaker feel more at ease, allowing him to speak more comfortably and honestly.

As a result, option C) use body language to understand how a speaker feels is the correct answer.

To learn more about Active listening visit:

https://brainly.com/question/3013119

#SPJ4

Answer:

Use body language to understand how a speaker feels

Edge 2023-2024

Other Questions
Identify two ways that congress regulates the postal service Imagine that you are planning to conduct field work and need to record earthquakes. What is the minimum number of seismometers you will need in order to locate the earthquakes?. use the properties of vector addition and scalar multiplication from the following theorem. properties of vector addition and scalar multiplication in rn let u, v, and w be vectors in rn, and let c and d be scalars. standard to slope intercept form Cathy has 48m of fencing to make a rectangular pen for her pets. What is themaximum area? 22 Which inequality is equivalent to 7x - 2y > 8?Fy> / x+885 y> - /x + Hy< x -34417 What will be the location of T in the image trapezoid STUV? (10, 1) (10, 1) (13, 1) (13, 1) several properties of water are shown classify the ones that are phycical propertys and the chemical propertys Why do yall not explain like please I went on brainly for a reason the anthropologist oscar lewis suggested the cause of poverty lies in group of answer choices a lower-class culture of poverty. lack of government action. too few available jobs. bad public schools. a fitness electronics company is a first-mover in wearable fitness trackers. its innovative everyday activity and exercise bracelet has created a monopoly for its product. executives are apprehensive about the company's ability to maintain its competitive advantage because they know that rival companies have competent research and development skills and access to marketing, sales knowhow, and manufacturing capabilities. these rivals are considered: 1. Consider the generic reaction:A + 2BC AH = -55 kJDetermine the amount of heat emitted when each amountof reactant completely reacts (assume that there is morethan enough of the other reactant).(a) 1 mol A(b) 2 mol A(c) 1 mol B(d) 2 mol B What is the role of RNA? Hello, may I please have some help on this practice question? Thank you. 4. Evaluate A car company wants to build awind-powered car that converts 100 percentof the mechanical energy in the wind to themechanical energy of the moving car.Explain why the company will fail. sc.7.P.11.2 please answer question 98 please!thanks Estimate the value of each quotient. Drag each division problem to the correct category shown below. Do not find the actual quotient when solving this problem.2,45612526263,012426,960571,59035Quotients between 10 and 100Quotients between 100 and 1,000helpppppppppppppp PLEASE HELP ME! QUICK! whats the phycical property of water experimentally, it is found that 3.4 moles of a molecule in gaseous form requires the addition of 454 j of energy at constant volume in order to raise its temperature by 8.4 k. calculate the molar heat capacity at constant volume for this gas.